Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709
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چکیده
منابع مشابه
Production Of Alkaline Protease From Bacillus licheniformis (NCIM 2044) And Media Optimisation For Enhanced Enzyme Production
Production of alkaline protease from Bacillus licheniformis (NCIM 2044) was studied and characterized. Of different cheap source media chosen, maximum production of 172.425 U/ml was obtained for 1% wheat bran containing media. The enzyme assay at various optimum pH and temperatures were also determined.
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in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کاملProduction of Extracellular Protease and Determination of Optimal Condition by Bacillus Licheniformis BBRC 100053 (RESEARCH NOTE)
The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...
متن کاملEffect of Medium Composition on Commercially Important Alkaline Protease Production by Bacillus licheniformis N-2
Protease production by alkalophilic B. licheniformis N-2 was investigated in 50 mL of the growth medium consisting of (in g/L): glucose 10.0, soybean meal 10.0, K2HPO4 3.0, MgSO4·7H2O 0.5, NaCl 0.5 and CaCl2·2H2O 0.5 at pH=10. Different carbon and nitrogen sources in the form of fine powder of organic, inorganic and defatted meals were studied to select the suitable substrate for alkaline prote...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2020
ISSN: 1475-2859
DOI: 10.1186/s12934-020-01307-2